PI-SceI DNA substrate top strand, PI-SceI DNA substrate bottom strand, ENDONUCLEASE PI-SCEI: Authors: Moure, C.M., Gimble, F.S., Quiocho, F.A. Deposit date: 2002-06-03: Release date: 2002-09-27: Last modified: 2011-07-13: Method: X-RAY DIFFRACTION (3.2 Å) Cite: Crystal structure of the intein homing endonuclease PI-SceI bound to its ...
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I hereby certify th at true co pi es of the SCEI Receiver' s Brief in Opposition to the Division of Enforcement's Motion for S umm ary Di spos ition , along w ith the Declaration of Ba rry J. Mandel in sup port th ereof, were served as fo llows on Ju ne 18, 2015: By Federal Exp ress (o n e copy) :
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An efficient method for constructing a recombinant adenovirus (Ad) vector, based on an in vitro ligation, has been developed. To insert the foreign gene into an adenoviral DNA, we introduced three unique restriction sites, I-CeuI, SwaI, and PI-SceI, into the E1 deletion site of the vector plasmid, which contains a complete E1, E3-deleted adenovirus type 5 genome.
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1. To isolate I-SceI homing endonuclease variants with increased specificity for the wild-type recognition sequence. 2. To generate I-SceI variants in which the DNA binding and/or DNA cleavage activities can be activated or deactivated in response to small molecules. This aim will create reagents that can be temporally and spatially regulated. 3.